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Fig. 4 | BMC Nephrology

Fig. 4

From: TRPP2 dysfunction decreases ATP-evoked calcium, induces cell aggregation and stimulates proliferation in T lymphocytes

Fig. 4

Analysis of p-ERK, p-mTOR, NFkB and MIF expression in ADPKD T lymphocytes. a ERK phosphorylation is increased in ADPKD compared with control cells (2.7 ± 0.6 for PKD2; 2.5 ± 0.7 for PKD1; 3 ± 0.8 for PKD; 0.95 ± 0.51 for RRT; 0.7 ± 0.26 for CTRL. PKD2, PKD1 and PKD vs. CTRL: ***p < 0.001). b The activation of mTOR is greater in ADPKD than in CTRL T lymphocytes (1.43. ± 0.25 for PKD2; 1.34 ± 0.2 for PKD1; 1.55 ± 0.21 for PKD; 0.65 ± 0.21 for RRT; 0.31 ± 0.24 for CTRL. PKD2, PKD1 and PKD vs. CTRL: ***p < 0.001). c ADPKD T lymphocytes show increased levels of NFkB protein compared with control cells (2.23. ± 0.61 for PKD2; 2.49 ± 0.68 for PKD1; 1.74 ± 0.40 for PKD; 0.89 ± 0.30 for RTT; 0.94 ± 0.27 for CTRL. PKD2, PKD1 and PKD vs. CTRL: **p < 0.01). d The expression of MIF is higher in ADPKD T lymphocytes than in controls (1.06 ± 0.12 for PKD2; 0.94 ± 0.20 for PKD1; 1.13 ± 0.17 for PKD; 0.23 ± 0.10 for RRT; 0.65 ± 0.13 for CTRL. PKD2 and PKD vs CTRL: **p < 0.01; PKD1 vs CTRL: *p < 0.05). CTRL = healthy controls; RRT = non-ADPKD subjects undergoing renal replacement therapy; PKD = non-genetically determined subjects; PKD1 = PKD1-related subjects; PKD2 = PKD2-related subjects. The phosphorylation levels were calculated as the ratio between band intensity of the phosphorylated form and total protein, while the expression of NFkB and MIF was calculated as ratio among the band relative to these proteins and beta-Actin. Data are expressed as mean ± standard deviation calculated from at least two different experiments in duplicate

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