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Fig. 1 | BMC Nephrology

Fig. 1

From: Superagonistic CD28 protects against renal ischemia injury induced fibrosis through a regulatory T-cell expansion dependent mechanism

Fig. 1

The experiment procedure and group division. IR (35-min ischemia) was induced by bilateral renal pedicles clamping followed by reperfusion. IPC was induced 6 days before IR for 18-min pre-ischemia. For the CD28sa-IR group, a bolus injection of 4–4.5 mg/kg CD28sa in 1 ml phosphate buffer (PBS) was administered intraperitoneally 6 days before IR. Apart from the IR procedure and CD28sa injection, 8–9 mg/kg of monoclonal anti-CD25 antibody (clone PC61, Biolegend, USA) was administered via peritoneal injection for the CD28sa-PC61-IR group on days 3 and 5 following the 35-min ischemia on day 7, for in vivo depletion of Tregs. IR, ischemia reperfusion; IPC, ischemia preconditioning; CD28sa, CD28 superagonists. Scr, serum creatinine; FCM, flow cytometry

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