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Fig. 2 | BMC Nephrology

Fig. 2

From: Superagonistic CD28 protects against renal ischemia injury induced fibrosis through a regulatory T-cell expansion dependent mechanism

Fig. 2

CD28sa promotes Tregs expansion and alleviates renal ischemic reperfusion injury. a. Serum creatinine (Scr) was significantly decreased in CD28sa treated mice from day 1 to day 3 post IRI. *P < 0.05, compared with the IR group (n = 6). b-d. Mice were sacrificed on day 7, 14, and 28 post-ischemia. Mononuclear cells from the peripheral blood, spleen, and kidney were obtained for various analyses. Flow cytometry was applied for the detection of CD4+ Foxp3+ Tregs and the percentage of Tregs from the total number of CD4+ T cells. The percentage of Tregs from peripheral blood, spleen, and kidney was much higher in the CD28sa pre-treated mice than that in the IR group from day 1 to day 7 post IRI. e. Flow cytometric detection of IL-17A+CD4+ T cells in kidneys. f. Quantitative analysis of Th17 cells in kidneys. g. Flow-cytometric detection of CD11c+MHCII+ dendritic cells in kidneys. h. Quantitative statistics of renal dendritic cells. *P < 0.05 compared with the IR group (n = 6). #P < 0.05 compared with the CD28sa-IR group (n = 6)

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BMC Nephrology

ISSN: 1471-2369

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