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Fig. 3 | BMC Nephrology

Fig. 3

From: Superagonistic CD28 protects against renal ischemia injury induced fibrosis through a regulatory T-cell expansion dependent mechanism

Fig. 3

CD28sa pretreatment retards CKD progression post-IRI. Following CD28sa or PC61 pretreatment 6 days before, an ischemia reperfusion injury was performed on day 0 and animals were killed for various analyses on day 7, 14, and 28. a. Representative images of hematoxylin-eosin (HE) stained kidney sections (original magnification × 200, Bar = 100 μM). b. Representative images of Masson-stained kidney sections (original magnification × 200, Bar = 100 μM). c. Representative images of Picosirus-red stained kidney sections (original magnification × 200, Bar = 100 μM). d. Immunoblot showed that CD28sa pretreatment significantly downregulated collagen IV expression of the kidneys at 28 days after IRI. e. Histogram represented the protein expression of Collage IV in mouse kidneys. These results were from 3 independent experiments, expressed as means ± SEM. *P < 0.05 compared with IR group (n = 6). #P < 0.05 compared with the CD28sa-IR group (n = 6)

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