Fig. 2

a-c. Electron microscopy images: a. Glomerulus with adjacent capillary loops lined by swollen endothelial cells and occluded by several electron-dense crystals with sharp edges, magnification X 1900, b. Glomerular capillary loop with an electron-dense occluding crystal, magnification X 6800; this loop shows moderate podocyte foot process effacement, while few other loops (not shown) showed severe foot process effacement, c. Image showing a higher magnification of the same crystal in b revealing a lattice-like substructure, magnification X 49000. d-f. Immunogold electron microscopy images: d. Control without primary antibody (X 5000), e. Labeling of crystal with numerous kappa gold particles (X 8000), f. Crystal with scant lambda gold particles (X 5000). μm = microns