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Fig. 4 | BMC Nephrology

Fig. 4

From: Brilliant blue G, a P2X7 receptor antagonist, attenuates early phase of renal inflammation, interstitial fibrosis and is associated with renal cell proliferation in ureteral obstruction in rats

Fig. 4

BBG reduces the renal collagen expression (protein and mRNA) in UUO. a Graphical representation of Picro-sirius Red surface density in renal cortex. The graphic shows aligned dot plot and the mean ± SEM of 20 captured images of each kidney in the different experimental groups. * p < 0.05 vs UUO-BBG and both SHAM groups. b Representative staining of the renal cortex from UUO-V group and c UUO-BBG group (Bar = 100 μm). d Graphical representation of Picro-sirius Red surface density in renal medulla. The graphic shows aligned dot plot and the mean ± SEM of 20 captured images of each kidney in the different experimental groups. * p < 0.05 vs UUO-BBG and both SHAM groups. e Representative staining of the renal medulla from UUO-V group and f UUO-BBG group. All pictures of the sham control groups are shown in the supplementary material. Bar = 100 μm in all Figs. (g) Graphical representation of the mRNA expression of Procollagen I by semiquantitative RT-PCR. Quantification of densitometric values obtained from the ratio Procollagen I /Ciclophilin (mean ± SEM, n = 6) under the 4 experimental conditions indicated in abscissa. * p < 0.05 vs UUO-BBG and both SHAM groups. # p < 0.05 vs UUO-V and both SHAM groups. h Graphical representation of the mRNA expression of Procollagen III by semiquantitative RT-PCR. Quantification of densitometric values obtained from the ratio Procollagen III/Ciclophilin (mean ± SEM, n = 5) under the 4 experimental conditions indicated in the abscissa. * p < 0.05 vs UUO-BBG and both SHAM groups. # p < 0.05 vs UUO-V and both SHAM groups. g Graphical representation of the mRNA expression of Procollagen IV by semiquantitative RT-PCR. Quantification of densitometric values obtained from the ratio Procollagen IV/Ciclophilin (mean ± SEM, n = 6) under the 4 experimental conditions indicated in the abscissa. * p < 0.05 vs UUO-BBG and both SHAM groups

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