Fig. 4From: Brilliant blue G, a P2X7 receptor antagonist, attenuates early phase of renal inflammation, interstitial fibrosis and is associated with renal cell proliferation in ureteral obstruction in ratsBBG reduces the renal collagen expression (protein and mRNA) in UUO. a Graphical representation of Picro-sirius Red surface density in renal cortex. The graphic shows aligned dot plot and the mean ± SEM of 20 captured images of each kidney in the different experimental groups. * p < 0.05 vs UUO-BBG and both SHAM groups. b Representative staining of the renal cortex from UUO-V group and c UUO-BBG group (Bar = 100 μm). d Graphical representation of Picro-sirius Red surface density in renal medulla. The graphic shows aligned dot plot and the mean ± SEM of 20 captured images of each kidney in the different experimental groups. * p < 0.05 vs UUO-BBG and both SHAM groups. e Representative staining of the renal medulla from UUO-V group and f UUO-BBG group. All pictures of the sham control groups are shown in the supplementary material. Bar = 100 μm in all Figs. (g) Graphical representation of the mRNA expression of Procollagen I by semiquantitative RT-PCR. Quantification of densitometric values obtained from the ratio Procollagen I /Ciclophilin (mean ± SEM, n = 6) under the 4 experimental conditions indicated in abscissa. * p < 0.05 vs UUO-BBG and both SHAM groups. # p < 0.05 vs UUO-V and both SHAM groups. h Graphical representation of the mRNA expression of Procollagen III by semiquantitative RT-PCR. Quantification of densitometric values obtained from the ratio Procollagen III/Ciclophilin (mean ± SEM, n = 5) under the 4 experimental conditions indicated in the abscissa. * p < 0.05 vs UUO-BBG and both SHAM groups. # p < 0.05 vs UUO-V and both SHAM groups. g Graphical representation of the mRNA expression of Procollagen IV by semiquantitative RT-PCR. Quantification of densitometric values obtained from the ratio Procollagen IV/Ciclophilin (mean ± SEM, n = 6) under the 4 experimental conditions indicated in the abscissa. * p < 0.05 vs UUO-BBG and both SHAM groupsBack to article page