Fig. 4
From: APOL1 renal risk variants exacerbate podocyte injury by increasing inflammatory stress
CAG-APOL1-B3 transgenic mice expressed APOL1-B3 in podocytes and tubular cells. a CAG (chicken ß-actin promoter) APOL1-B3-FLAG transgenic mice were generated in a FVB/N mouse background. To confirm the expression of APOL1-B3 in the kidney, extracts from whole kidneys were subjected to Western analysis using indicated antibodies, showing that APOL1-B3 was expressed. b Localization of APOL1-B3 in the kidney was determined by immunohistochemistry using rabbit monoclonal antibody (EPR2907). Podocalyxin was used as a podocyte marker. Scale bar shows 40 μm