Fig. 6
From: APOL1 renal risk variants exacerbate podocyte injury by increasing inflammatory stress
APOL1-B3 and C isoforms uniquely interacted with NLRP12. Shown are the structure of FLAG-tagged APOL1 isoforms expression vectors. Also shown is the design of the NLRP12 expression vector highlighting the three major domains (pyrin domain, PYD; NACHT domain, and leucine rich repeat, LRR) of this protein. The constructs were transiently transfected into HeLa cells. APOL1-B3 and APOL1-C isoforms co-immunoprecipitated with NLRP12, while the secreted APOL1-B1 isoform did not. APOL1-B3 interacted with NLRP12 but not APOL1-B1, which is identical to APOL1-A in its amino acid sequence