Fig. 7From: Constitutive Atg5 overexpression in mouse bone marrow endothelial progenitor cells improves experimental acute kidney injuryAnalysis of the p62 expression in CD31+ (endothelial) cells. As in previous reports by our group, the autophagocytic flux was evaluated by p62 staining. IRI alone did not elevate endothelial p62. If native of transfected cells were applied however, endothelial p62 increased at both, 48 h and 6 weeks. Images b-e show kidney sections from one Control group (48 h), f-i display sections from an animal that received transfected EPCs (48 h). b + f: staining of p62 exclusively; c + g: staining of CD31 exclusively; d + h: merge; e + i: magnifications of d and h. (magnifications in b-d and in f-h × 40; in e and i ∼ × 60; green: p62; red: CD31; blue: nuclei; Data in A as mean +/− SEM; *: p-values between certain groups of the ‘48 h cohort’ < 0.05 – the bracketed lines indicate comparisons between two groups; #: p-values between different groups of the ‘6 weeks cohort’ < 0.05 – the bracketed lines indicate respective comparisons)Back to article page