Fig. 5

Immunostaining of ACE2 in the diabetic mouse kidney with Pg-LPS-induced nephropathy. Hematoxylin-Eosin staining (HE) (left column); immunostaining for ACE2 (red) (center column); and merged immunostaining for ACE2 and podoplanin (green) (right column), with DAPI staining of nuclei (blue). The glomerular epithelial cells are immunostained by anti-podoplanin to be able to discriminate glomeruli (arrows). Reaction with anti-ACE2 (arrowheads) was only observed in the proximal tubular cells with brush borders but not in any other region including distal tubules in LPS-ICR (top row) or in STZ-ICR (middle row) while the reaction was identified in proximal and distal tubules, and in glomeruli (yellow arrowheads) in LPS-STZ (bottom row). Podoplanin-positive macrophages were also identified in LPS-STZ (yellow arrow). Bars: 100 μm