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Fig. 1 | BMC Nephrology

Fig. 1

From: Higher CD19+CD25+ Bregs are independently associated with better graft function in renal transplant recipients

Fig. 1

a Gating strategy for CD19 + CD25+ Bregs. (1) After ensuring the stability of the run and (2) exclusion of doublets, (3) lymphocytes were gated according to size. (4) Subsequently, CD19 was plotted versus CD45 to identify CD19+ cells. CD25 was then plotted against Foxp3 to further characterize CD19 + CD25+ Bregs into Foxp3- and Foxp3 + . b Gating strategy for Tregs. All the events were analyzed after (1) ensuring stability of the run and (2) exclusion of doublets. (3) Lymphocytes were gated according to size. (4) Afterwards, CD45 was gated to focus on lymphocytes. (5) CD25 vs CD4 dot plot allows the identification of CD4 + CD25+ T lymphocytes, (6) Foxp3 + CD127- cells were gated out of CD4 + CD25+ lymphocytes to identify Tregs

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BMC Nephrology

ISSN: 1471-2369

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