Fig. 4From: A disease-causing variant of COL4A5 in a Chinese family with Alport syndrome: a case seriesIn vivo validation of the splicing of COL4A5 gene. A Total RNA was obtained from the fat tissue, and was analyzed by RT-PCR. 1, DNA ladder; 2, RT-PCR product. B RT-PCR product was directly sequenced. Black line indicated the additional fragmentBack to article page