Fig. 3

miR-543 negatively regulates TSPAN8 in HK2 cells. A The predicted miR-543 binding sites on the 3’UTR of TSPAN8 was shown. B Dual luciferase reporter assay was performed using vectors containing WT binding site or mutated binding site between miR-543 and TSPAN8, with the empty vector (Luci-NC) as the negative control. Vectors were co-transfected with miR-NC or miR-543 mimic. C-D The mRNA and protein levels of TSPAN8 were decreased or increased in HK2 cells after the transfection with miR-543 mimic or miR-543 inhibitor. *P < 0.05, **P < 0.01 or ***P < 0.001