Fig. 3

miR-466a-3p mediated the protective effect of PPS on AGEs-induced fibrosis and inflammation. A Expression of miRNAs was determined by qRT-PCR in SV40 RES13 cells treated with AGEs or PPS + AGEs for 24 h. B The miR-446-3p negative control (NC) or inhibitor was transfected into SV40 RES13 cells treated with AGEs and PPS. The expression of miRNAs was determined by qRT-PCR. C Western blot analyses of PIK3CA. The miR-446-3p negative control (NC) or inhibitor was transfected into SV40 RES13 cells for 48 h. The protein level of PIK3CA was determined by western blot. D Western blot analyses of TGF-β1 and FN. The miR-446-3p negative control (NC) or inhibitor was transfected into SV40 RES13 cells treated with AGEs and PPS for 48 h. The protein levels of TGF- β1 and FN were determined by western blot. E The miR-446-3p negative control or inhibitor was transfected into SV40 RES13 cells treated with AGEs and PPS for 48 h. The protein levels of IL-6 and TNFα were determined by ELISA assay. All the experiments were performed in triplicate, and the results were analyzed by one-way ANOVA or Student’s t-test (unpaired, two-tailed). The data are expressed as mean ± SEM.