Fig. 4

miR-466a-3p inhibited PI3K/AKT signaling pathway via PIK3CA in AGEs-treated SV40 MES13 cells. A Schematic graph of the putative binding sites of miR-466a-3p in the PIK3CA 3′-UTR. Mut 3′-UTR indicates the PIK3CA 3′-UTR with a mutation in the miR-466a-3p binding sites. B miR-466a-3p mimics downregulated the activity of a luciferase reporter containing wild-type PIK3CA 3′-UTR but not of a reporter containing mutant PIK3CA 3′-UTR. C Forty-eight hours after miR-466a-3p control and inhibitor transfection of SV40 RES13 cells treated with AGEs and PPS, the protein levels of PIK3CA, p-PI3K, pAKT, and AKT were determined by Western blot analysis. GAPDH was used as the internal control. All the experiments were performed in triplicate. The results were analyzed by one-way ANOVA (more than two groups) or Student’s t-test (unpaired, two-tailed, two groups). The data are expressed as mean ± SEM.