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Fig. 5 | BMC Nephrology

Fig. 5

From: METTL3 enhances NSD2 mRNA stability to reduce renal impairment and interstitial fibrosis in mice with diabetic nephropathy

Fig. 5

METTL3-YTHDF1 regulates the stability of NSD2 mRNA. A m6A level in SV40-MES-13 cells with METTL3 overexpression; B m6A modification level on NSD2 mRNA in SV40-MES-13 cells with METTL3 overexpression; C NSD2 mRNA stability in SV40-MES-13 cells with METTL3 overexpression; D Abundance of NSD2 mRNA enriched by YTHDF antibodies examined by RIP-qPCR; E–F, mRNA (E) and protein (F) levels of YTHDF1 and NSD2 in cells after YTHDF1 silencing examined by RT-qPCR and western blot analysis; G, NSD2 mRNA stability in SV40-MES-13 cells with YTHDF1 inhibition. Data were expressed as the mean ± SD from three independent experiments. Differences were analyzed by the unpaired t test (A-B) or two-way ANOVA (C-G). *p < 0.05

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BMC Nephrology

ISSN: 1471-2369

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