Fig. 4
From: Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy
The SRGAP2a expressions and actin cytoskeleton of podocytes following HG and HG plus klotho treatment. A The immunofluorescence staining of SRGAP2a with HG and HG plus klotho treatment. B The qRT-PCR results showing SRGAP2a mRNA expressions with HG and HG plus klotho treatment. C The western blotting results showing SRGAP2a protein expressions with HG and HG plus klotho treatment. D The quantitative analysis of the SRGAP2a protein levels in C. E The fluorescein-conjugated phalloidin staining of podocytes with HG and HG plus klotho treatment. F The quantitative analysis of the mean actin content per cell in E. All data were mean ± SD, (n = 6); *p < 0.05, **p < 0.01. Bars = 50 μm in A, 5 μm in E. NC, negative controls without additional treatment