Fig. 4

The expression levels of inflammatory factors in different Lee’s grade of IgAN children. Expression of MCP-1 (A), IL-17 (B), IL-6 (C) and TNF-α (D) in kidney tissue of control, and low grade group (Lee’s grade I to II) (n = 7, middle column) and high grade group (grade III to IV) (n = 8, right column) was detected by DAB staining. The Bar in A-D was 50 µm. Image J analysis software was used to calculate the relative optical density (OD) in control group, low and high grade group (E). Compared with control group, *P < 0.05, **P < 0.01; Comparison between Lee’s grade groups, ^#P < 0.05, ^{# #}P < 0.01