Demographic, diagnostic and therapeutic characteristics of autosomal dominant polycystic kidney disease in Ghana

Background Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the commonest of the hereditary kidney diseases and mostly ensues in utero with signs delayed until after several decades. This study assessed the demographic, diagnostic (clinical and biochemical features) and therapeutic patterns among ADPKD patients who attended the nephrology unit of Komfo Anokye Teaching Hospital (KATH) from 2007 to 2018. Methods This cross-sectional retrospective analysis of ADPKD patient records was conducted at the nephrology unit of KATH in October 2020. The records of 82 ADPKD was used for this study. Demographic, clinical, biochemical, ultrasonographic and therapeutic data was obtained, organized and analyzed with Statistical Package for the Social Sciences (SPSS). Results ADPKD was most prevalent in people within the ages of 31–40 years (25.6 %), with a male (52.4 %) preponderance. The most common clinical features presented were flank pain (30.5 %) and bipedal swelling (18.3 %). Hypertension (42.7 %), urinary tract infections (UTIs) (19.5 %), and anemia (13.4 %) were the most common complications reported. Average level of HDL-c was higher in females (1.7) than in males (1.2) (p = 0.001). Hematuria (34 %) and proteinuria (66 %) were among the biochemical derangements presented. About 81.7 % had CKD at diagnosis with the majority in stages 1 (27.0 %), 3(23.2 %) and 5 (20.3 %). Poor corticomedullary differentiation was observed in 90.2 % of participants and increased echogenicity was observed in 89.0 % of the participants. Estimated GFR (eGFR) correlated positively with echotexture (r = 0.320, p = 0.005) and negatively with CMD (r= -0.303, p = 0.008). About 95.1 % of patients were on conservative therapy including: 73.2 %, 52.4 %, 22.0 %, 13.4 %, 8.5 % on Irebesartan/Lisinopril, Nifecard XL, Hydralazine, Methyldopa and Bisoprolol respectively for hypertension; 26.8 and 3.7 % on Gliclazide and Metformin respectively for Type 2 diabetes mellitus; 25.6 %, 24.4 and 18.3 % on CaCO3, fersolate and folic acid respectively as nutrient supplements with 4.9 % of participants on renal replacement therapy (RRT). Conclusions ADPKD occurs in people aged ≥ 31 years with a higher male preponderance. Clinical features include flank and abdominal pain, bipedal swelling, headache, amongst others. Uremia, hematuria, proteinuria, decreased eGFR, were the common biochemical derangements reported with higher severity detected in men. The therapeutic interventions mostly involved conservative therapy to manage symptoms and other comorbid conditions and rarely renal replacement therapy (RRT).


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Conclusions: ADPKD occurs in people aged ≥ 31 years with a higher male preponderance. Clinical features include flank and abdominal pain, bipedal swelling, headache, amongst others. Uremia, hematuria, proteinuria, decreased eGFR, were the common biochemical derangements reported with higher severity detected in men. The therapeutic interventions mostly involved conservative therapy to manage symptoms and other comorbid conditions and rarely renal replacement therapy (RRT).
Keywords: Autosomal dominant polycystic kidney disease, Chronic kidney disease, Demographic characteristics, Clinical features, Biochemical derangements Background Autosomal Dominant Polycystic Kidney Disease (ADPK D) is the most common form of hereditary kidney disease that mostly manifests during adulthood [1]. Characterized by the formation and growth of multiple cysts, ADPKD distorts kidney structure and function and ultimately results in End Stage Kidney Damage (ESRD) [2]. The genetic basis of the condition is a mutation in the polycystin genes (PKD1 and PKD 2) and rarely, the recently detected GANAB [3] and PMM2 [4] genes. Disease course is often associated with other extra-renal disease characteristics including cyst formation in different organs such as the spleen, liver, pancreas, etc.; abdominal wall hernias; cardiovascular abnormalities among others which contribute to disease morbidity [5]. It is a lifelong progressive condition and until recently, there was no ADPKD specific treatment [6,7].
ADPKD is the fourth highest cause of CKD in Ghana [8] and accounts for about 5-10 % of ESRD patients worldwide [9]. In Europe, it is noted as the fourth kidney disease diagnosis that requires renal replacement therapy (RRT), with an estimate of about one tenth of all RRT patients having ADPKD [6].
Diagnosis of the ADPKD is based on family history, clinical presentations and radiological imaging. A positive family history; presence of clinical features such as: hematuria, back pain etc. and radiologic proof of bilateral fluid-filled renal cysts confirms the disease [9]. In the developed world, advanced genetic testing is also employed in the screening and diagnosis of ADPKD [10].
The diagnostic and therapeutic characteristics of the disease need to be clearly defined especially in our context. In Africa, only few studies have been conducted on ADPKD making information on ADPKD specific for Africans especially Ghanaians very scarce. With this scarcity of data, we are compelled to rely on information from advanced countries for the diagnosis and management of our patients. Differences in geographical location, genetic predispositions, climatic conditions, economic and infrastructural resources, sometime makes this inappropriate. It is therefore necessary to provide population-specific information regarding ADPK D especially in Ghana. In light of the above we sought to assess the demographic, diagnostic (clinical and biochemical) and therapeutic patterns among ADPKD patients who attended the nephrology unit of KATH, Ghana.

Study site/ study design
This cross-sectional retrospective study of patient records was conducted at the nephrology unit of Komfo Anokye Teaching Hospital (KATH) during the period of 2007 to 2018. KATH is the second largest hospital in Ghana with 1000 bed capacity and a well-resourced nephrology unit. The nephrology clinic is an adult (for ages 18 years and above) nephrology clinic, with services sometimes extending to patients as young as 14 years of age.

Eligibility criteria
Records of patients diagnosed with ADPKD after clinical, imaging and biochemical testing within the period under review was used. Records of participants with other renal diseases and those with incomplete data were excluded.

Participants/collection of retrospective data
Data of 82 ADPKD patients (male/female) was manually collected from the records of the nephrology unit. The period of data collection was from 2007 to 2018. Demographic information of each participant including gender, age, geographical location and occupation was collected. Clinical information obtained included type of renal disease presented, presence or absence of hypertension, other non-renal diseases presented, results of biochemical tests and treatment administered to the patient and ultrasound results.

Ethical consideration
Ethical clearance for the study was obtained from the research and development unit of KATH. Data used was obtained in adherence to the principles of the declaration of Helsinki and local regulatory requirements.

Data analysis
Data was analyzed using Statistical Package for the Social Sciences (SPSS), version 22.0. Test for normality was performed with box plot, kurtosis and Kolmogorov-Smirnoff test. Descriptive summary statistics such as frequencies, percentages and bar charts were presented as appropriate. Parametric data were presented as means ± standard deviation and median (interquartile ranges) for non-parametric data. The Student's T test or the Mann-Whitney U test was used appropriately to test the descriptive statistics for continuous variables and the Chi square test for categorical variable was used whenever applicable. Relationships between clinical parameters were assessed with Pearson and Spearman rank tests. The statistical significance of variables were set at p value of < 0.05 and 95 % confidence level.  Table 2).

Results
The baseline clinical laboratory findings are summarized in Table 3. The mean systolic pressure was 140.8 ± 26.9 mm Hg and mean diastolic pressure was   (Table 4).

Discussion
We assessed the demographic, diagnostic and therapeutic characteristics of ADPKD patients in Nephrology clinic at KATH, Kumasi. In line with earlier studies, we found that the disease mostly presented in patients aged 31 years and above, with the highest occurrence within ages 31 to 40 years [1,11,12]. This is consistent with existent literature that ADPKD often occurs in adults over 30 years of age [2,13,14]. Similar to the studies of Arogundade et al., [15] and Chijioke et al., [16] we detected a male preponderance in disease incidents.
In accordance with Hajji et al., [1], the drugs reported in this study are designed to manage the symptoms, complications and comorbidities associated with the disease. According to Pei et al., [17], the development of hypertension indicates disease progression and must be managed ideally with blood pressure target of 130/80 mm/Hg. It is not surprising that most of our study participants presented with hypertension. The activation of RAAS and sympathetic nervous system are the predominant causes of hypertension in ADPKD. Left ventricular hypertrophy (LVH) also causes high BP and increases cardiovascular risk in this condition [1]. Therefore, early detection and treatment of hypertension could hinder cyst development and cardiovascular complications [18]. Angiotensin converting enzyme inhibitors (ACEI)s and Angiotensin receptor blockers (ARBs) are the preferred medications used in the management of hypertension in ADPKD because they have been proven beneficial to: control cardiac dysfunction; decrease LVH and end organ damage; and reduce mortality [14]. Hence it is not surprising that most of our participants were on Lisinopril and Irbesartan. Again, majority of our participants (95.1 %) were on conservative therapy with very few (4.9 %) on dialysis. Usually in CKD, patients are managed for as long as possible and hemodialysisusually the last resortis started when GFR has receded to < 15 mL/min [19].
Bipedal swelling and flank pain are common symptoms reported in patients with ADPKD. In consonance with the study of Mandal et al., [20], we observed bipedal swelling and flank pain among our participants. Bipedal swelling usually occurs due to the lower serum  oncotic pressure from the nephrotic proteinuric range characteristic of kidney disease whereas flank pain results directly and indirectly from cyst formation and enlargement on kidneys and sometimes, nearby organs such as the liver [21]. Flank pain was reported by a rather lower percentage (30.5 %) of our participants than that (68.3 %) reported by Arogundade et al., [15], most likely due to our larger sample size. UTIs, were the second highest complication (19.5 %) at diagnosis. Other studies reported much higher percentages with female predominance [15,22,23]. With about 97.6 % of our participants having CKD and 15.9 % of them already in end stage, it is typical that anemia was reported in (13.5 %) although this was rather a low perecentage. Some of these patients may have polycythemia resulting from cystic production of erythropoietin hence preventing anemia progression. Most of the patients seem to have normal potassium levels although the levels in males were significantly higher than in females. Typical of CKD and in accordance with the findings of Helal et al., [24] and Arogundade et al., [15] urea and creatinine levels were high for most of the participants. With our study, urea was particularly higher in males than in females. These findings concede with the fact that disease progression is suggested to be faster in men than in women [25]. Others attribute hormonal differencescontributing to disease pathogenesisto the better prognosis seen in men than in women [26]. With HDL-c levels significantly lower in males than in females, we can infer that cardiovascular risk is higher for males than for females in our study.
From the correlation analysis, eGFR was negatively correlated with CMD and with 97.6 % of our participants having CKD, it is normal to have 90.2 % of them showing poor CMD upon imaging (Fig. 2). Loss of CMD associated with decreasing eGFR, is characteristic of renal  insufficiency resulting from edema, renal atrophy, renal scarring, etc. [27,28]. The positive correlation between eGFR and echogenicity we found is expected, since increased kidney echogenicity usually signifies an underlying kidney disease. Similar to the findings of Cristea et al., [29], kidney size (defined by the LPP) was significantly high (averagely 15.56 cm for right kidney and 15.47 cm for left - Table 5), most likely due to multiple cyst formation and enlargement in the kidney.
As observed in our study (Table 6), proteinuria and hematuria are common observations in ADPKD [30]. Whereas hematuria in ADPKD mostly occurs either due to hemorrhage into cysts or cyst rapture into collecting ducts, proteinuria occurs due to impaired reabsorption of proteins by cyst-lined tubular epithelia [31] but is not usually observed in the early stages [32].

Limitations
Our study has the following limitations. First, data used was collected from one nephrology clinic and follow up information was not documented. Second, most of the study participants were lost to follow up, hence survival analysis could not be well performed,although, with the data we had, only two of our participants died due to causes not known to us.Third, there is no such data present in the country, hence we cannot conclusively draw comparative inferences on patient outcomes. Fourth, due to the inability to detect ADPKD early in disease course [5], milder forms of the disease may have been excluded.

Conclusions and recommendations
In Ghana ADPKD mostly occurs in people of age 31 years and above with a higher male preponderance. Clinical features include flank and abdominal pain, bipedal swelling, headache, amongst others. Uremia, hematuria, proteinuria and decreased eGFR are some of the biochemical derangements presented by patients with this condition. Hypertension turned out to be the most common comorbidity as well as complication associated with ADPKD with proteinuria, hematuria, UTIs, anemia amongst others developing as disease progresses. The therapeutic interventions mostly involve conservative therapy to manage symptoms and other comorbid conditions and rarely renal replacement therapy (RRT). Early diagnosis of the disease should be targeted by engaging more advanced ultrasonography. Also, molecular diagnosis may be employed for early diagnosis in high-risk individuals such as those with a family history of the disease. Future researchers may explore multicenter studies and engage proper monitoring regimens to analyze disease outcomes in our part of the world,