A novel model of adenine-induced tubulointerstitial nephropathy in mice

BMC Nephrology

Table 1 Histopathological evaluation of kidneys from control and adenine-treated mice

Compartment	Parameter	Control group	Adenine group
Compartment	Parameter	(n = 5)	(n = 8)
Glomeruli	Sclerosis (present/absent)	0/5	0/8
Glomeruli	Dilatation of Bowmans capsules (present/absent)	0/5	5/3
Tubuli	Rounded cristalloid/amorphous structures in tubular lumina (present/absent)	0/5	8/0
	Cell debris/necrotic material and PMN in tubular lumina (0–3)	0 (0–0)	1 (1–1)
	Tubular atrophy (0–3)	0 (0–0)	2 (2–3)
	Thyroidization (0–3)	0 (0–0)	1 (1–2)
	Focal dilatation of tubuli (present/absent)	0/5	6/2
	Focal calcium deposits (present/absent)	0/5	5/3
Interstitium	Fibrosis (0–3)	0 (0–0)	1 (1–1)
Interstitium	Inflammation (0–3)	0 (0–0)	0 (0–1)
Vessels	Morphology (pathological/normal)	0/5	0/8

The parameters tubular atrophy, cell debris/necrotic material and polymorphonuclear leukocytes (PMN) in tubular lumina, thyroidization, interstitial fibrosis and interstitial inflammation are graded as follows: 0; affecting 0-5% of the renal area, 1; 6-25%, 2; 26-50%, and 3; >50%. Data is presented as median (range). Rounded cristalloid/amorphous structures in tubular lumina, focal dilatation and focal calcium deposits in tubuli are graded as present or absent. Vessel morphology is graded as pathological or normal.

ISSN: 1471-2369