Figure 3

Glomerular and tubulointerstitial expression of collagen I 20 weeks after induction of chronic anti-thy1 glomerulosclerosis (cGS). Representative photographs of immunhistochemically stained sections show a normal control animal (A), an animal with uninephrectomy (B), an animal with anti-thy1-induced cGS without (C) and with Imatinib (D) treatment. Graphs depict the percentages of collagen I-positive staining of at least 15 randomized tubulointerstitial (E) and glomerular sections (F) obtained by computer-based histomorphometry. Administration of Imatinib was started 7 days after injection of anti-thy1 antibody into uni-nephrectomized rats (cGS + Imatinib). Nonnephritic animals with (1-K Control) and without uninephrectomy (2-K Control) received an injection with similar volumes of PBS. Magnification ×200. (*P < 0.05 and ***P < 0.001 vs. cGS).