Figure 3

Knockdown of p65 alleviates indoxyl sulfate-induced decrease of Nrf2 in HK-2 cells. A: HK-2 cells were transfected with or without NF-κB p65 siRNA (10 nM), and then serum starved for 24 h. Whole cell lysates were immunoblotted using anti-Nrf2 antibody. B: HK-2 cells were transfected with or without NF-κB p65 siRNA (10 nM), and then serum starved for 24 h, followed by indoxyl sulfate (250 μM) for 48 h. Expression levels of Nrf2 mRNA were measured by real-time PCR. Data are expressed as mean±SE (n=4). *P < 0.05 vs. indoxyl sulfate-treated cells without p65 siRNA. C: experimental conditions were as described in B except treatment with indoxyl sulfate for 72 h was used in place of 48 h. Whole cell lysates were immunoblotted using anti-Nrf2 antibody.