Figure 7
Representative immunohistochemical detection of CAT (first row), Cu, Zn-SOD (second row), and Mn-SOD (third row) during acute renal failure induced by K2Cr2O7. The study was performed in control rats (day 0) and on days 2 and 12 after a single injection of K2Cr2O7 (15 mg/Kg). (A) Normal kidney from control rats shows intense CAT immunoreactivity in the proximal and distal convoluted tubules. (B) In contrast, kidney cortex on day 2 shows evident decrease of CAT immunostaining. (C) Strong CAT immunoreactivity in the tubular epithelium is observed on day 12. (D) Normal kidney from control animals also showed strong Cu, Zn-SOD immunostaining in the cortex convoluted tubules. This strong Cu, Zn-SOD immunoreactivity is also observed on day 2 (E) and on day 12 (F). A similar pattern of Mn-SOD immunostaining is observed in normal kidney from control rat (G), and after 2 (H) and 12 (I) days of K2Cr2O7 injection. A-C and G-I 100X, D-F 400X.