Skip to main content
Fig. 5 | BMC Nephrology

Fig. 5

From: Resveratrol attenuates constitutive STAT3 and STAT5 activation through induction of PTPε and SHP-2 tyrosine phosphatases and potentiates sorafenib-induced apoptosis in renal cell carcinoma

Fig. 5

RES potentiates the cytotoxic and apoptotic effects of sorafenib in renal cell carcinoma. a and b Caki-1 and 786-O cells (5× 103 cells/well) were treated with indicated concentration of RES, and sorafenib for 24 h. The cytotoxicity was determined by MTT assays (left panel). The combination index (CI) values were obtained using BiosoftCalcuSyn software (Biosoft, Cambridge, UK) (right panel). c Caki-1 and 786-O cells (1 × 106 cells/well) were treated with indicated concentration of RES, and sorafenibfor6h. After which whole-cell extracts were prepared and 15 μg portions of those extracts were resolved on 8 % SDS-PAGE gel, electrotransferred onto nitrocellulose membranes, and probed against p-STAT3(Tyr705), p-STAT3(Ser727), STAT3, p-STAT5(Tyr694/699), and STAT5. The results shown here are representative of three independent experiments. d and e Caki-1 and 786-O cells (1 × 106 cells/well) were treated with indicated concentration of RES, and sorafenibfor24h. Whole-cell extracts were prepared, and 20 μg of the whole-cell lysate was resolved by SDS-PAGE, electrotransferred to nitrocellulose membrane, and then probed with antibodies against bcl-2, bcl-xL, survivin, caspase-3, and PARP. The same blots were stripped and reprobed with β-actin antibody to verify equal protein loading

Back to article page

BMC Nephrology

ISSN: 1471-2369

Contact us