Fig. 3

Differentiation antagonizing non-protein coding ribonucleic acid (RNA) (DANCR) functioned as a microRNA (miR)-214-5p sponge in diabetic nephropathy (DN). A. Subcellular localization analysis of DANCR in human kidney 2 cells. B. Bioinformatics found potential binding sites between DANCR and miR-214-5p. The specificity target between DANCR and miR-214-5p was explored using dual luciferase reporter assays (C) and RNA immunoprecipitation assay (D). E. Quantitative reverse transcription polymerase chain reaction was used to identify the expression of serum miR-214-5p in the participants. F. Correlation analysis of DANCR levels and miR-214-5p in patients with DN. G. Expression of miR-214-5p in high glucose (HG)-induced and DANCR-suppressed HK-2 cells. *** P < 0.001 vs. health volunteer group or control; ### P < 0.001 vs. diabetes mellitus group or HG + mall interfering-negative control