Fig. 5

Differentiation antagonizing non-protein coding ribonucleic acid (RNA) (DANCR) modulates Krüppel-like factor 5 (KLF5) expression by competitively binding to microRNA (miR)-214-5p. A. Potential binding sites of miR-214-5p to KLF5. B. Dual luciferase reporter assay was adopted to examine the effect of miR-214-5p levels on the luciferase activity of KLF-5- wild type and KLF5-mutant. C. RNA immunoprecipitation assay was performed to analyze the enrichment between miR-214-5p, DANCR, and KLF5. D. Effect of miR-214-5p levels on KLF5 protein levels detected by Western blot. E. The KLF5 expression in the serum of the participants was analyzed. Correlation analysis of serum KLF5 levels with miR-214-5p (F) and DANCR (G) levels in patients with diabetic nephropathy, respectively. H. KLF5 levels after DANCR knockdown and inhibition of miR-214-5p in high glucose (HG)-induced HK-2 cells. *** P < 0.001 vs. healthy volunteer group or control; ### P < 0.001 vs. HG + small interfering negative control; & P < 0.05, && P < 0.01, &&& P < 0.001