Sericin cream reduces pruritus in hemodialysis patients: a randomized, double-blind, placebo-controlled experimental study
© Aramwit et al.; licensee BioMed Central Ltd. 2012
Received: 6 February 2012
Accepted: 22 September 2012
Published: 24 September 2012
Uremic pruritus (UP) is a significant complication in ESRD patients and substantially impairs their quality of life. UP is considered to be a skin manifestation of chronic inflammation. Because sericin can suppress the release of pro-inflammatory cytokines, the purpose of this study was to investigate the short-term safety and efficacy of sericin cream for treating UP in hemodialysis patients.
This study used a double-blind design to investigate the effects of random topical administration of sericin cream and cream base (placebo) on either the right or left extremities of hemodialysis patients for 6 weeks. Skin hydration, irritation and pigmentation were evaluated every 2 weeks using Skin Diagnostic SD27. The visual analog scale for itching was also evaluated every 2 weeks, and the Kidney Disease Quality of Life Short Form was performed on the day of each patient’s enrollment and after 6 weeks of treatment.
Fifty dialysis patients were enrolled, 47 of which completed the study. The hydration of the skin of the patients’ extremities increased significantly after administration of sericin cream; significant differences were found between sericin treatment and control after 6 weeks of treatment (p = 0.041 for arms and p = 0.022 for legs, respectively). Moreover, a significant difference was also found in skin irritation between the two treatments (p = 0.013 for arms and p = 0.027 for legs, respectively). At the end of the study, the skin pigmentation level was significantly reduced on both the arms (p = 0.032) and legs (p = 0.021) of the sericin-treated side compared with the side treated with cream base. The mean itching score decreased significantly from moderate to severe at the time of enrollment to mild pruritus after 6 weeks of treatment (p = 0.002). A better quality of life was found in all domains tested although statistically significant differences before and after treatment was found only in the patients’ pain scores, the effect of kidney disease on daily life, sleep quality and symptoms or problems related to kidney disease.
We conclude that sericin cream has a high potential for reducing UP in hemodialysis patients.
The trial registration number of this study is ISRCTN16019033; its public title is “sericin cream reduces pruritus in hemodialysis patients”.
KeywordsPruritus Hemodialysis Sericin Hydration Inflammation Pigmentation
Itching associated with end-stage renal disease (ESRD) or uremic pruritus (UP) affects between 20 and 50% of renal failure patients [1–3] who have no primary skin disease or systemic or psychological dysfunction that might cause pruritus. In addition, approximately 80% of patients undergoing hemodialysis were found to be affected by UP [4–6]. Unfortunately, dialysis has only a slight impact on pruritus .
Pruritus is an unpleasant symptom that negatively impacts a patient’s quality of life. The impact of moderate/severe uremic pruritus on the mortality of patients with ESRD seems to be associated with sleep disturbance rather than with uremic pruritus per se[8, 9]. Moreover, in the recent Dialysis Outcomes and Practice Patterns Study II (DOPPS II) trial, pruritus was associated with depression, sleep disturbance and increased mortality risk [8, 10]. The pathophysiology of UP is still unclear; many hypotheses have been proposed to explain its occurrence, including xerosis and hypohidrosis (a condition in which the skin is usually atrophic and dry), the presence of pruritogenic cytokines (histamine, kallikrein, interleukin [IL]-2, acetylcholine and other substances that are released by histamine-mediated mast cell stimulation and may lower the UP threshold), secondary hyperparathyroidism, immune-inflammatory reactions in which sericin cream is thought to play a central role in producing an anti-inflammatory effect, the uremic neuropathy hypothesis and the opioid hypothesis (k-opioid receptor understimulation and overexpression of μ-opioid receptors) [7, 8].
Sericin, a biopolymer with a high molecular weight, is a water-soluble protein that is obtained from the silkworm (Bombyx mori) . Sericin is characterized by the presence of 32% serine, which is the main amino acid of the natural moisture factor (NMF) in human skin; therefore, sericin has excellent moisturizing properties that may be helpful for treating hypohidrosis. Sericin also shows many biological activities and has been widely studied for potential use in medicines and biomaterials [12–16]. In 2009, Aramwit et al. showed that sericin significantly decreased the levels of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in sericin-treated wounds in rats 7 days after an injury compared with the levels found in normal saline-soaked wounds and cream base-treated wounds . As previously mentioned, the immune-inflammatory hypothesis considers UP a dermatologic manifestation of chronic inflammation and treats the condition as a possible result of derangements in the immune system that are based on a pro-inflammatory pattern. Based on this reasoning, sericin may help to relieve UP.
At present, there is no widely accepted treatment for UP. Topical products, such as moisturizers and emollients, are typically used to alleviate symptoms. Because sericin can suppress the release of pro-inflammatory cytokines, the present study was designed to investigate the short-term safety and efficacy of sericin cream for treating UP in ESRD patients. The quality of life of ESRD patients after using sericin cream was also evaluated.
Molecular weight and amino acid composition of sericin
Amino acid composition of sericin
Aspartic acid (Asp)
Glutamic acid (Glu)
Baseline characteristics of subjects (N = 47)
49.6 ± 11.2
Dialysis duration (months)
24.6 ± 3.1
30.12 ± 3.55
57.89 ± 12.65
10.98 ± 2.25
9.87 ± 1.32
4.35 ± 1.02
3.96 ± 0.62
14.23 ± 5.98
15.32 ± 5.12
Alkaline phosphatase (IU/L)
96.58 ± 40.32
Total bilirubin (mg/dL)
0.32 ± 0.11
Itching (scale 1–10)
7.05 ± 2.17
Sericin cream treatment
Cream base treatment
28.67 ± 7.11
27.55 ± 7.84
25.10 ± 7.67
23.29 ± 7.37
409.34 ± 232.09
318.50 ± 484.26
364.74 ± 120.20
308.22 ± 116.14
373.56 ± 142.32
360.35 ± 249.29
435.94 ± 178.06
402.25 ± 154.53
Skin parameters of hydration, irritation and pigmentation on the subjects’ extremities after treatment
Skin irritation was measured based on the redness of the skin; both the sericin cream and the cream base reduced the level of skin irritation throughout the study period. As shown in Table 3, the arms and legs that were treated with sericin cream showed statistically significantly reduced irritation or redness from the second week after treatment until the end of the study compared to the baseline (p = 0.031 for arms and p = 0.040 for legs). The degree of redness of the legs of the patients who were treated with the cream base during weeks 4 (p = 0.048) and week 6 (p = 0.036) was also significantly reduced compared to the baseline. In addition, a significant difference between treatment with sericin cream and cream base was found in the level of skin irritation of both arms (p = 0.013) and legs (p = 0.027) after 6 weeks of treatment.
The use of sericin cream significantly reduced the darkness of the skin on both the arms and legs of the patients, as shown in Table 3. In addition, in the sixth week of treatment, there was a significant difference in the level of skin pigmentation in the arms and legs treated with the sericin cream compared to those treated with the cream base (Figure 1 and 2, p = 0.032 for arms and p = 0.021 for legs, respectively). The cream base showed no statistically significant effect on the level of skin pigmentation, and no significant difference compared to baseline was found in the skin pigmentation of the patients’ arms and legs after use of the cream base for 6 weeks (p = 0.082 for arms and p = 0.067 for legs, respectively).
Mean scores for each domain of the KDQOL-SF in hemodialysis patients on the enrollment day and after 6 weeks of treatment (N = 47)
After treatment for 6 weeks
Physical component score (PCS)
73.90 ± 18.99
77.93 ± 18.57
50.00 ± 42.20
51.83 ± 44.16
48.29 ± 16.96
74.27 ± 17.36*
43.05 ± 24.08
47.32 ± 22.17
Mental component score (MCS)
50.73 ± 18.93
56.59 ± 17.62
69.82 ± 20.53
78.35 ± 22.54
56.10 ± 42.45
61.79 ± 41.87
61.17 ± 15.60
64.98 ± 16.51
Kidney disease component score (KDCS)
64.33 ± 9.98
78.51 ± 10.44*
Effect of kidney disease on daily life
59.91 ± 20.46
68.22 ± 16.33*
Burden of kidney disease
43.45 ± 29.38
44.21 ± 25.29
65.53 ± 18.25
69.27 ± 19.43
60.98 ± 41.10
68.29 ± 39.93
71.74 ± 33.12
75.00 ± 23.62
Quality of social interaction
70.08 ± 18.80
76.91 ± 16.06
43.41 ± 22.08
52.74 ± 18.22*
70.33 ± 22.52
76.02 ± 25.01
Dialysis staff encouragement
83.84 ± 13.76
87.50 ± 12.18
70.73 ± 20.34
78.05 ± 17.65
60.00 ± 21.68
61.95 ± 19.90
This study has shown that the use of sericin cream can reduce UP in hemodialysis patients according to their VAS scores. Sericin cream can also significantly increase skin hydration and reduce skin irritation and skin pigmentation in patients. The molecular weight analysis of the sericin used in this study gave results that agree with those reported by Sprague, who indicated that sericin is a mixture of at least 15 different polypeptide chains ranging in size from 20–220 kDa . Similar to the earlier report by Kato et al., serine was the most abundant amino acid found in sericin .
Although antihistamines, anti-allergic agents and topical corticosteroids are commonly used to treat UP in ESRD patients, their effectiveness is sometimes limited [2, 8, 21, 22]. Alternative and effective treatments for intractable pruritus that generate low levels of adverse reactions, particularly in sensitive patients such as the ESRD group, must therefore be developed. This clinical study is the first to suggest that a sericin cream is beneficial for treating pruritus in hemodialysis patients. The benefits of this treatment apparently result from increases in skin hydration and suppression of pro-inflammatory cytokines, as shown in our earlier study [17, 23], thereby resulting in less skin irritation without any allergic reactions.
Pruritus associated with chronic kidney disease has been shown to be associated with elevated levels of C-reactive protein and other inflammatory cytokines. This evidence suggests that there is an inflammatory component in this form of pruritus . Some authors consider UP to be a skin manifestation of chronic inflammation . Recently, the “persistent microinflammation” theory, in which it is suggested that inflammation may be related to the genesis of UP, was proposed . The skin of ESRD patients with UP contains an increased number of mast cells, and these cells can release various substances such as histamine, interleukins (IL) and tumor necrosis factor (TNF) [27–30].
Pruritic skin can appear normal except for dryness; because the severity of pruritus is closely correlated with skin dryness , xerosis treatment normally begins with a topical agent such as a gentle moisturizing cream. Because sericin is well known for its moisturizing effect and its ability to reduce the generation of pro-inflammatory cytokines by fibroblasts, this study assessed the usefulness of sericin cream in treating UP. Evaluating the degree of pruritus was difficult because it is a subjective symptom; therefore, functional measurements of skin conditions known to be associated with pruritus, such as skin hydration and skin irritation, were considered to be objective indices. We found that both sericin cream and the cream base used to prepare the sericin cream increased the moisture content of the stratum corneum; however, the level of hydration was significantly higher in the skin treated with sericin cream. The side of the body treated with sericin cream had significantly lower levels of post-treatment dryness compared to pretreatment dryness and lower total dermatological severity scores in parameters such as skin pigmentation.
The pathogenesis of ESRD itself is associated with inflammation and oxidative stress, as reflected in studies of plasma biochemistry, including the levels of the cytokines IL and TNF-α [32–34], classic markers of inflammatory processes that are strongly affected by pathological conditions . These cytokines are upregulated in the plasma of ESRD patients [32, 36]. The skin inflammation associated with the release of IL and TNF-α is further complicated by the inflammatory lesions that occur secondary to scratching . Our results indicate that improvement in skin hydration and suppression of the release of pro-inflammatory cytokines in skin might be the mechanism by which sericin improves UP. Skin irritation, which can be caused by either skin inflammation or by scratching, was substantially reduced in the sericin group compared with the cream base group after 6 weeks of treatment. This result confirmed that sericin reduces skin irritation in patients with UP and suggested that it might be due to suppression of the inflammatory cytokines in skin. Similar results have been obtained in animal studies .
Secondary skin lesions that can result from the itch-scratch cycle include excoriations, hyperpigmentation, lichenification, prurigo nodules and scars . Chronic renal failure is usually accompanied by a variety of cutaneous manifestations; dermal manifestations, including hyperpigmentation, have also been reported, and these can exact a considerable toll on the quality of life [39–41]. Although skin pigmentation is not related to specific clinical symptoms, it has an important effect on patient satisfaction. Sericin has been reported to have activity against tyrosinase , an enzyme related to melanin production; as this study shows, treatment with sericin results in significant reduction in the skin color of patients.
The pruritus/mortality relationship may be strongly attributed to sleep disturbances, as previously mentioned in the DOPPS [8, 9, 42]. Many previous studies have found a strong association between inflammation, as measured by C-reactive protein or inflammatory cytokines, and sleep disturbances in dialysis patients [43–45]. The significant effects of pruritus on sleep, mood and social functioning require further investigation with the goal of improving the available treatments for this serious ESRD complication. We found that reduction in itching intensity from moderate to severe pruritus at the time of enrollment to mild pruritus after 6 weeks of treatment was associated with a better quality of life in all of the measured domains, including the mental, physical and kidney disease components. The KDQOL-SF score in the ESRD patients indicated improvements in several domains regarding the patients’ quality of life, particularly pain and sleep, which are relevant to itching. A better quality of sleep may reflect some degree of relief from itching achieved through the treatments.
This study has certain limitations. First, the study design was an in-subject controlled study; it cannot be determined whether the improvements in the itching evaluation and the quality of life were from the sample (sericin cream) or the placebo (cream base). Moreover, due to the in-subject controlled design, the biochemical parameters could not be evaluated at the end of the study. Second, the study included a small number of patients, and these were followed for a relatively short time. A long-term study with a larger sample size is necessary. In addition to studies of hemodialysis patients, similar studies of other ESRD patients, such as peritoneal dialysis and kidney transplant patients, are also necessary to confirm the findings presented here.
Our study shows that sericin reduces pruritus in patients with UP. The use of sericin cream significantly increased the level of skin hydration after 6 weeks of treatment compared to baseline and to the use of the cream base. The use of sericin cream also significantly reduced the level of skin irritation and pigmentation after 6 weeks of treatment compared to baseline, while use of the cream base reduced skin pigmentation slightly but not significantly. The results of this study suggest that sericin cream may be a good choice for treating pruritus in hemodialysis patients.
Preparation of silk sericin cream
Because commercial sericin cream is unavailable, the sericin cream used in this study was prepared from raw materials. Bombyx mori cocoons were purchased from Chul Thai Silk Co., Ltd. (Petchaboon province, Thailand). The cocoons were extracted with purified water (1 g of dry silk cocoon: 30 mL of water) using a high temperature and pressure degumming technique in an autoclave (SS-320, Tomy Seiko Co., Ltd., Tokyo, Japan) at 121°C and 15 psi for 60 min. This technique has been shown to be safe for the preparation of material used on keratinocyte and fibroblast cells and can activate high collagen production related to wound healing . After filtration through a membrane (Whatman filter paper No. 1, Whatman PLC, Kent, United Kingdom) to remove fibroin, sericin powder was obtained by freezing and lyophilizing the sericin solution with a Heto LL3000 lyophilizer (Allrod, Denmark). Petroleum jelly, mineral oil, lanolin, glycerin, bisabolol, triethanolamine stearate, propylparaben and methylparaben were used to formulate a cream base. For an 8% sericin cream, a concentration that has been shown to be safe and effective in the treatment of second-degree burn wounds, the sericin powder was dissolved in warm water and then mixed with the other ingredients during the cream-forming process.
Molecular weight determination of sericin
To determine the molecular weight of sericin, SDS-PAGE was performed as previously described, with some modifications . Briefly, samples were prepared for SDS-PAGE by adding an equal volume of sample buffer (0.25 M Tris–HCl, pH 7.0 containing 4% SDS, 10% sucrose, 10% 2-mercaptoethanol and 0.025% bromophenol blue) to each protein solution. Each sample was then incubated at 98°C for 2–3 min and loaded onto a 5%-20% gradient gel (Atto Corporation, Tokyo, Japan). Electrophoresis was performed in 125 mM Tris base with 0.96 M glycine and 0.5% SDS, and the polypeptide bands were detected using silver staining.
Amino acid analysis of sericin
The amino acid composition of sericin was determined using an amino acid analyzer (Hitachi L-8500A, Tokyo, Japan). Samples for analysis were hydrolyzed in 4 M methanesulfonic acid containing 0.2% 3-(2-aminoethyl) indole (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) at 100°C for 24 h under vacuum. The experiments were performed in triplicate.
Skin hydration may be related to pruritus, and this parameter is sensitive to relative humidity, personal activities and diet. Therefore, an in-subject control using a split-body biometrological assessment (each patient received both treatments but on different sides of the body) was used to evaluate the safety and efficacy of the sericin cream for the treatment of UP in hemodialysis patients. Moreover, the distribution of pruritus between the patients was highly variable whereas the manifestation of mirror symmetry was an attribute they all shared . An in-subject, randomized, double-blind, placebo-controlled experimental study was designed to investigate the effects of the sericin cream versus the cream base (placebo) in reducing the symptoms of UP (itching, dryness and redness) and skin pigmentation in stable maintenance hemodialysis patients. Each of the parameters, including skin hydration, skin irritation, skin pigmentation and itching score, was evaluated at baseline and at 2, 4 and 6 weeks after treatment intervention. The sericin cream and the cream base were identical in texture and scent. All of the products were packaged in containers that were label-free except for the treatment code number, and the packages were identical in shape, size and color; therefore, the treatment assignment remained unknown to the participants, the study investigators and the medical personnel. The subjects were recruited from December 2010 to February 2011, and the study was conducted between March 2011 and December 2011 at the Division of Nephrology, Phramongkutklao Hospital and at Priest Hospital, Thailand. Signed informed consent was obtained from all subjects after a thorough discussion of the protocol, its rationale and the potential risks. This study was approved by the Ethics Committee of the Institute Review Board at Phramongkutklao Hospital, Thailand and ended after the last participant completed the intervention.
All ESRD patients at Phramongkutklao and Priest Hospitals over 18 years of age who had received hemodialysis for at least 3 months were screened for this study. Having mild to severe pruritus as measured by the VAS during the previous 6 weeks was also an inclusion criterion. The patients were required to refrain from using any antipruritic treatment (oral or topical) for a period of not less than 2 weeks prior to the start of the study. Patients of both genders, regardless of comorbidities or prescribed medications, were eligible. Any medication that had an antipruritic effect was discontinued 2 weeks before the study. No changes in the patients’ prescription medications were required during this study with the exception of a concomitant antipruritic treatment.
Pruritus caused by other skin diseases or medication was excluded by careful clinical assessment. Patients with a history of silk protein allergy, who were allergic to any compounds in the formula, or who had biliary atresia, liver problems, cancer, metabolic disorders or other diseases related to systemic pruritus were also excluded. Patients who had skin problems or rashes on their extremities (arms or legs) were also excluded from this study. Participants left the project when they could not comply with the treatment, when they were unwilling to continue with the study, or when the physician opined that other treatments were needed to relieve the symptoms. After reviewing patient profiles and explaining the protocol, 27 patients were excluded due to liver problems (N = 4), cancer (N = 3), metabolic disorder (N = 7), rashes on their extremities (N = 6) and refusal to participate in the study (N = 7). The remaining patients (N = 50) were enrolled in the study.
In 2004, Okada and Matsumoto  evaluated the effect of an emollient containing a high water content on mild uremic pruritus; based on this study, the number of samples needed for a dependent sample was approximately 50 subjects. Split-body biometrological assessments were performed. The physician investigator enrolled the subjects into this study, and using a computer-generated block of four, another investigator generated the random allocation sequence that divided the patients into two groups. The identities of the patients in each group were concealed from both the investigators and the patients. The on-duty nurses assigned the participants to the intervention. The participants, investigators and those assessing the outcomes were blinded after assignment to the interventions. The patients in the first group received the sericin cream on their left extremities (left arm and left leg), while the other side of the body received the cream base. The patients in the second group received both the sericin cream and the cream base, but on opposite sides of the body from the first group. All of the patients were shown how to topically apply the assigned treatment evenly over the area indicated twice daily for a period of 6 weeks after showering.
Measurement and outcome
where P0 is the value of each parameter at baseline (at the time of enrollment) and Pt is the value of each parameter during the follow-up period (2, 4 or 6 weeks). All of the measurements were performed in triplicate.
Because itching is a systemic symptom and likely to be generalized, most patients could not identify whether the itching occurred primarily on the right or left side of the body; therefore, itching was scored as an overall symptom. The severity of itching was systemically assessed on both the arms and legs of all patients using VAS on the enrollment day and every 2 weeks after treatment began. We used a VAS that consisted of a 10-cm horizontal line with no scale markings. The patients were asked to mark the intensity of their itching on the scale, with the strongest possible level of itching or unbearable pruritus marked on the right end of the line (10 cm) and no itching marked on the left end (0 cm) .
Patients’ quality of life
The patients’ quality of life was assessed using the Thai version of the KDQOL-SF Version 1.3 . Quality of life was evaluated on the enrollment day and after 6 weeks of treatment. The mean scores for the individual domain scores and for the three composite summary scores, which include the mental component score (MCS), the physical component score (PCS) and the kidney disease component score (KDCS), were compared as shown in Table 4. For the Hayes algorithm , the raw data obtained from the patients were first transformed into a pre-coded numeric value of 0–100; a higher transformed score reflected a better quality of life [51, 53].
The occurrence of allergic reactions during treatment with sericin cream was regularly evaluated by two dermatologists during each visit. The Naranjo algorithm was used to determine the likelihood of whether an adverse drug reaction was actually caused by the sericin cream or by other factors.
The results are expressed as the mean ± SD unless otherwise indicated. Statistical analysis was performed using SPSS version 10.0 (SPSS Inc., Chicago, Illinois, USA). A bidirectional α-level of significance was set at p = 0.05 for all of the measurements. From the baseline to weeks 2, 4 and 6, the VAS score changes and the levels of skin hydration, irritation and pigmentation were computed for each patient within the treatment group using a repeated measure analysis of variance (ANOVA). The paired t-test was used to analyze changes in the patients’ quality of life between baseline and 6 weeks after treatment. The differences in each parameter for the patients receiving the sericin cream and the cream base were compared at each time point using Student’s t-test.
This research was funded by the Thailand Research Fund, which is a government-operated, non-profit organization for research support.
- Feramisco JD, Berger TG, Steinhoff M: Innovative management of pruritus. Dermatol Clin. 2010, 28 (3): 467-478. 10.1016/j.det.2010.03.004.View ArticlePubMedGoogle Scholar
- Patel TS, Freedman BI, Yosipovitch G: An update on pruritus associated with CKD. Am J Kidney Dis. 2007, 50 (1): 11-20. 10.1053/j.ajkd.2007.03.010.View ArticlePubMedGoogle Scholar
- Mettang M, Weisshaar E: Pruritus: control of itch in patients undergoing dialysis. Skin Therapy Lett. 2010, 15 (2): 1-5.PubMedGoogle Scholar
- Chen YC, Chiu WT, Wu MS: Therapeutic effect of topical gamma-linolenic acid on refractory uremic pruritus. Am J Kidney Dis. 2006, 48 (1): 69-76. 10.1053/j.ajkd.2006.03.082.View ArticlePubMedGoogle Scholar
- Urbonas A, Schwartz RA, Szepietowski JC: Uremic pruritus–an update. Am J Nephrol. 2001, 21 (5): 343-350. 10.1159/000046272.View ArticlePubMedGoogle Scholar
- Virga G, Visentin I, La Milia V, Bonadonna A: Inflammation and pruritus in haemodialysis patients. Nephrol Dial Transplant. 2002, 17 (12): 2164-2169. 10.1093/ndt/17.12.2164.View ArticlePubMedGoogle Scholar
- Akhyani M, Ganji MR, Samadi N, Khamesan B, Daneshpazhooh M: Pruritus in hemodialysis patients. BMC Dermatol. 2005, 5: 7-10.1186/1471-5945-5-7.View ArticlePubMedPubMed CentralGoogle Scholar
- Pisoni RL, Wikstrom B, Elder SJ, Akizawa T, Asano Y, Keen ML, Saran R, Mendelssohn DC, Young EW, Port FK: Pruritus in haemodialysis patients: International results from the Dialysis Outcomes and Practice Patterns Study (DOPPS). Nephrol Dial Transplant. 2006, 21 (12): 3495-3505. 10.1093/ndt/gfl461.View ArticlePubMedGoogle Scholar
- Wikstrom B: Itchy skin–a clinical problem for haemodialysis patients. Nephrol Dial Transplant. 2007, 22 (Suppl 5): v3-7.View ArticlePubMedGoogle Scholar
- Narita I, Alchi B, Omori K, Sato F, Ajiro J, Saga D, Kondo D, Skatsume M, Maruyama S, Kazama JJ, et al: Etiology and prognostic significance of severe uremic pruritus in chronic hemodialysis patients. Kidney Int. 2006, 69 (9): 1626-1632. 10.1038/sj.ki.5000251.View ArticlePubMedGoogle Scholar
- Zhang YQ: Applications of natural silk protein sericin in biomaterials. Biotechnol Adv. 2002, 20 (2): 91-100. 10.1016/S0734-9750(02)00003-4.View ArticlePubMedGoogle Scholar
- Aramwit P, Sangcakul A: The effects of sericin cream on wound healing in rats. Biosci Biotechnol Biochem. 2007, 71 (10): 2473-2477. 10.1271/bbb.70243.View ArticlePubMedGoogle Scholar
- Zhaorigetu S, Yanaka N, Sasaki M, Watanabe H, Kato N: Inhibitory effects of silk protein, sericin on UVB-induced acute damage and tumor promotion by reducing oxidative stress in the skin of hairless mouse. J Photochem Photobiol B. 2003, 71 (1–3): 11-17.View ArticlePubMedGoogle Scholar
- Zhaorigetu S, Yanaka N, Sasaki M, Watanabe H, Kato N: Silk protein, sericin, suppresses DMBA-TPA-induced mouse skin tumorigenesis by reducing oxidative stress, inflammatory responses and endogenous tumor promoter TNF-alpha. Oncol Rep. 2003, 10 (3): 537-543.PubMedGoogle Scholar
- Aramwit P, Damrongsakkul S, Kanokpanont S, Srichana T: Properties and antityrosinase activity of sericin from various extraction methods. Biotechnol Appl Biochem. 2010, 55 (2): 91-98. 10.1042/BA20090186.View ArticlePubMedGoogle Scholar
- Aramwit P, Siritientong T, Kanokpanont S, Srichana T: Formulation and characterization of silk sericin-PVA scaffold crosslinked with genipin. Int J Biol Macromol. 2010, 47 (5): 668-675. 10.1016/j.ijbiomac.2010.08.015.View ArticlePubMedGoogle Scholar
- Aramwit P, Kanokpanont S, De-Eknamkul W, Srichana T: Monitoring of inflammatory mediators induced by silk sericin. J Biosci Bioeng. 2009, 107 (5): 556-561. 10.1016/j.jbiosc.2008.12.012.View ArticlePubMedGoogle Scholar
- Gupta MA, Gupta AK, Kirkby S, Weiner HK, Mace TM, Schork NJ, Johnson EH, Ellis CN, Voorhees JJ: Pruritus in psoriasis. A prospective study of some psychiatric and dermatologic correlates. Arch Dermatol. 1988, 124 (7): 1052-1057. 10.1001/archderm.1988.01670070040016.View ArticlePubMedGoogle Scholar
- Sprague KU: The Bombyx mori silk proteins: characterization of large polypeptides. Biochemistry. 1975, 14 (5): 925-931. 10.1021/bi00676a008.View ArticlePubMedGoogle Scholar
- Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M: Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem. 1998, 62 (1): 145-147. 10.1271/bbb.62.145.View ArticlePubMedGoogle Scholar
- Schwartz IF, Iaina A: Uraemic pruritus. Nephrol Dial Transplant. 1999, 14 (4): 834-839. 10.1093/ndt/14.4.834.View ArticlePubMedGoogle Scholar
- Mettang T, Pauli-Magnus C, Alscher DM: Uraemic pruritus–new perspectives and insights from recent trials. Nephrol Dial Transplant. 2002, 17 (9): 1558-1563. 10.1093/ndt/17.9.1558.View ArticlePubMedGoogle Scholar
- Aramwit P, Kanokpanont S, Punyarit P, Srichana T: Effectiveness and Inflammatory Cytokines Induced by Sericin Compared to Sericin in Combination with Silver Sulfadiazine Cream on Wound Healing. Wounds. 2009, 21 (8): 198-206.PubMedGoogle Scholar
- Berger TG, Steinhoff M: Pruritus and renal failure. Semin Cutan Med Surg. 2011, 30 (2): 99-100. 10.1016/j.sder.2011.04.005.View ArticlePubMedPubMed CentralGoogle Scholar
- Duque MI, Thevarajah S, Chan YH, Tuttle AB, Freedman BI, Yosipovitch G: Uremic pruritus is associated with higher kt/V and serum calcium concentration. Clin Nephrol. 2006, 66 (3): 184-191.View ArticlePubMedGoogle Scholar
- Kimmel M, Alscher DM, Dunst R, Braun N, Machleidt C, Kiefer T, Stulten C, van der Kuip H, Pauli-Magnus C, Raub U, et al: The role of micro-inflammation in the pathogenesis of uraemic pruritus in haemodialysis patients. Nephrol Dial Transplant. 2006, 21 (3): 749-755. 10.1093/ndt/gfi204.View ArticlePubMedGoogle Scholar
- Lugon JR: Uremic pruritus: a review. Hemodial Int. 2005, 9 (2): 180-188. 10.1111/j.1492-7535.2005.01130.x.View ArticlePubMedGoogle Scholar
- Dugas-Breit S, Schopf P, Dugas M, Schiffl H, Rueff F, Przybilla B: Baseline serum levels of mast cell tryptase are raised in hemodialysis patients and associated with severity of pruritus. J Dtsch Dermatol Ges. 2005, 3 (5): 343-347. 10.1111/j.1610-0387.2005.05706.x.View ArticlePubMedGoogle Scholar
- Szepietowski J, Thepen T, van Vloten WA, Szepietowski T, Bihari IC: Pruritus and mast cell proliferation in the skin of haemodialysis patients. Inflamm Res. 1995, 44 (Suppl 1): S84-85.View ArticlePubMedGoogle Scholar
- Matsumoto M, Ichimaru K, Horie A: Pruritus and mast cell proliferation of the skin in end stage renal failure. Clin Nephrol. 1985, 23 (6): 285-288.PubMedGoogle Scholar
- Young AW, Sweeney EW, David DS, Cheigh J, Hochgelerenl EL, Sakai S, Stenzel KH, Rubin AL: Dermatologic evaluation of pruritus in patients on hemodialysis. N Y State J Med. 1973, 73 (22): 2670-2674.PubMedGoogle Scholar
- Pereira BJ, Shapiro L, King AJ, Falagas ME, Strom JA, Dinarello CA: Plasma levels of IL-1 beta, TNF alpha and their specific inhibitors in undialyzed chronic renal failure, CAPD and hemodialysis patients. Kidney Int. 1994, 45 (3): 890-896. 10.1038/ki.1994.117.View ArticlePubMedGoogle Scholar
- Stenvinkel P, Ketteler M, Johnson RJ, Lindholm B, Pecoits-Filho R, Riella M, Heimburger O, Cederholm T, Girndt M: IL-10, IL-6, and TNF-alpha: central factors in the altered cytokine network of uremia–the good, the bad, and the ugly. Kidney Int. 2005, 67 (4): 1216-1233. 10.1111/j.1523-1755.2005.00200.x.View ArticlePubMedGoogle Scholar
- Bolton CH, Downs LG, Victory JG, Dwight JF, Tomson CR, Mackness MI, Pinkney JH: Endothelial dysfunction in chronic renal failure: roles of lipoprotein oxidation and pro-inflammatory cytokines. Nephrol Dial Transplant. 2001, 16 (6): 1189-1197. 10.1093/ndt/16.6.1189.View ArticlePubMedGoogle Scholar
- Portugal-Cohen M, Oron M, Ma'or Z, Boaz M, Shtendik L, Biro A, Cernes R, Barnea Z, Kazir Z, Kohen R: Noninvasive skin measurements to monitor chronic renal failure pathogenesis. Biomed Pharmacother. 2011, 65 (4): 280-285. 10.1016/j.biopha.2011.02.001.View ArticlePubMedGoogle Scholar
- Khan SB, Cook HT, Bhangal G, Smith J, Tam FW, Pusey CD: Antibody blockade of TNF-alpha reduces inflammation and scarring in experimental crescentic glomerulonephritis. Kidney Int. 2005, 67 (5): 1812-1820. 10.1111/j.1523-1755.2005.00279.x.View ArticlePubMedGoogle Scholar
- Ponticelli C, Bencini PL: Uremic pruritus: a review. Nephron. 1992, 60 (1): 1-5. 10.1159/000186696.View ArticlePubMedGoogle Scholar
- Wang H, Yosipovitch G: New insights into the pathophysiology and treatment of chronic itch in patients with end-stage renal disease, chronic liver disease, and lymphoma. Int J Dermatol. 2010, 49 (1): 1-11. 10.1111/j.1365-4632.2009.04249.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Bencini PL, Montagnino G, Citterio A, Graziani G, Crosti C, Ponticelli C: Cutaneous abnormalities in uremic patients. Nephron. 1985, 40 (3): 316-321. 10.1159/000183485.View ArticlePubMedGoogle Scholar
- Avermaete A, Altmeyer P, Bacharach-Buhles M: Skin changes in dialysis patients: a review. Nephrol Dial Transplant. 2001, 16 (12): 2293-2296. 10.1093/ndt/16.12.2293.View ArticlePubMedGoogle Scholar
- Dyachenko P, Shustak A, Rozenman D: Hemodialysis-related pruritus and associated cutaneous manifestations. Int J Dermatol. 2006, 45 (6): 664-667. 10.1111/j.1365-4632.2005.02592.x.View ArticlePubMedGoogle Scholar
- Chiu YL, Chen HY, Chuang YF, Hsu SP, Lai CF, Pai MF, Yang SY, Peng YS: Association of uraemic pruritus with inflammation and hepatitis infection in haemodialysis patients. Nephrol Dial Transplant. 2008, 23 (11): 3685-3689. 10.1093/ndt/gfn303.View ArticlePubMedGoogle Scholar
- Yang JY, Huang JW, Chiang CK, Pan CC, Wu KD, Tsai TJ, Chen WY: Higher plasma interleukin-18 levels associated with poor quality of sleep in peritoneal dialysis patients. Nephrol Dial Transplant. 2007, 22 (12): 3606-3609. 10.1093/ndt/gfm231.View ArticlePubMedGoogle Scholar
- Chiu YL, Chuang YF, Fang KC, Liu SK, Chen HY, Yang JY, Pai MF, Peng YS, Wu KD, Tsai TJ: Higher systemic inflammation is associated with poorer sleep quality in stable haemodialysis patients. Nephrol Dial Transplant. 2009, 24 (1): 247-251.View ArticlePubMedGoogle Scholar
- Chen HY, Chiang CK, Wang HH, Hung KY, Lee YJ, Peng YS, Wu KD, Tsai TJ: Cognitive-behavioral therapy for sleep disturbance in patients undergoing peritoneal dialysis: a pilot randomized controlled trial. Am J Kidney Dis. 2008, 52 (2): 314-323. 10.1053/j.ajkd.2008.03.012.View ArticlePubMedGoogle Scholar
- Aramwit P, Kanokpanont S, Nakpheng T, Srichana T: The effect of sericin from various extraction methods on cell viability and collagen production. Int J Mol Sci. 2010, 11 (5): 2200-2211. 10.3390/ijms11052200.View ArticlePubMedPubMed CentralGoogle Scholar
- Takasu Y, Yamada H, Tsubouchi K: Isolation of three main sericin components from the cocoon of the silkworm, Bombyx mori. Biosci Biotechnol Biochem. 2002, 66 (12): 2715-2718. 10.1271/bbb.66.2715.View ArticlePubMedGoogle Scholar
- Mathur VS, Lindberg J, Germain M, Block G, Tumlin J, Smith M, Grewal M, McGuire D: A longitudinal study of uremic pruritus in hemodialysis patients. Clin J Am Soc Nephrol. 2010, 5 (8): 1410-1419. 10.2215/CJN.00100110.View ArticlePubMedPubMed CentralGoogle Scholar
- Okada K, Matsumoto K: Effect of skin care with an emollient containing a high water content on mild uremic pruritus. Ther Apher Dial. 2004, 8 (5): 419-422. 10.1111/j.1526-0968.2004.00175.x.View ArticlePubMedGoogle Scholar
- Wahlgren CF, Ekblom A, Hagermark O: Some aspects of the experimental induction and measurement of itch. Acta Derm Venereol. 1989, 69 (3): 185-189.PubMedGoogle Scholar
- Homjean K, Sakthong P: Translation and cognitive testing of the Thai version of the kidney disease quality of life short-form questionnaires version 1.3. Thai J Pharm Pract. 2010, 2 (1): 3-14.Google Scholar
- Park HJ, Kim S, Yong JS, Han SS, Yang DH, Meguro M, Han CW, Kohzuki M: Reliability and validity of the Korean version of Kidney Disease Quality of Life instrument (KDQOL-SF). Tohoku J Exp Med. 2007, 211 (4): 321-329. 10.1620/tjem.211.321.View ArticlePubMedGoogle Scholar
- Al Jumaih A, Al-Onazi K, Binsalih S, Hejaili F, Al-Sayyari A: A Study of Quality of Life and its Determinants among Hemodialysis Patients Using the KDQOL-SF Instrument in One Center in Saudi Arabia. Arab J Nephrol Transplant. 2011, 4 (3): 125-130.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2369/13/119/prepub