Figure 2
From: Bone Morphogenetic Protein (BMP)-7 expression is decreased in human hypertensive nephrosclerosis
Immunofluorescence of BMP-7 and Aquaporin 1 in normal kidneys revealing expression in distal tubules and proximal tubular cells. Double immunofluorescence of BMP-7 with aquaporin-1 (AQ-1): A) AQ-1 is green (FITC), B) BMP-7 staining is red (rhodamine red), C) double labelling of BMP-7 and AQ-1 is orange and nuclei is blue (DAPI). Parallel binding of BMP-7 and AQ-1 is seen mainly in tubules with smaller cuboidal cells and larger lumen indicating expression in distal tubules (small arrow) and less in proximal tubule cells (bulky arrow). Staining was low in the glomeruli. Original magnification: × 200. D) Negative control × 100.