Figure 8
From: Bone Morphogenetic Protein (BMP)-7 expression is decreased in human hypertensive nephrosclerosis
Real time PCR indicating reversal of TGF-β induced EMT by BMP-7. The mRNA level of the epithelial marker ZO-1 and E-cadherin and the mesenchymal marker α-sm-actin and S100A4 were analysed after stimulation of HK-2 cells with TGF-β alone, BMP-7 alone or both in combination (n = 3). There was a decrease of ZO-1 and E-cadherin and an increase of α-sm-actin and S100A4 mRNA after stimulation with TGF-β alone, while no significant effect was seen after stimulation with BMP-7 alone. Co-stimulation of TGF-β and BMP-7 led to a significant increase of ZO-1 (p < 0.001) and E-cadherin (p = 0.05) as well as a decrease of α-sm-actin (p < 0.001) and S100A4 mRNA (p < 0.05) compared to TGF-β alone. The results were analysed by Student's t-tests and are displayed as % of unstimulated cells.