Figure 4

Effects of doxycycline (Dox) on the development of PKD in PCK rats. A to C. Representative kidney sections from the vehicle-treated control (A) and PCK (B) rats as well as the doxycycline-treated PCK rat (C) (Scale bar, 1.5 cm). D. Doxycycline reduced the cyst volume of the PKD kidneys. The cyst volume density (CVD) of the kidneys from the vehicle-treated control (+/+ Veh) and PCK (PKD Veh) rats, and from the doxycycline-treated control (+/+ Dox) and PCK (PKD Dox) rats was measured. The doxycycline treatment did not affect CVD in +/+rats but decreased it in the PCK rats. *P < 0.001 versus +/+ vehicle, **p < 0.005 versus PKD vehicle. E. Doxycycline reduced the kidney enlargement in PCK rats. The two-kidney/total body weight ratio (2 K/TBW) was examined in the vehicle-treated control (+/+ Veh) and PCK (PKD Veh) rats and were compared to that of doxycycline-treated control (+/+ Dox) and PCK (PKD Dox) rats, respectively. The doxycycline treatment did not affect 2 K/TBW in +/+rats but decreased it in the PCK rats. *p < 0.001 versus +/+vehicle, **p < 0.005 versus PKD vehicle. F. Doxycycline treatment did not significantly affect creatinine level. The creatinine levels were measured in the vehicle-treated control (+/+Veh) and PCK (PKD Veh) rats and were compared to those in the doxycycline-treated control (+/+ Dox) and PCK (PDK Dox) rats. *p < 0.001 versus +/+ vehicle. G. BUN was unaffected by doxycycline in +/+rats. The PCK rats at 9 weeks of age have a slight increase in BUN (*p < 0.005 versus +/+ vehicle); however, the doxycycline treatment did not significantly affect BUN level.