Figure 4
From: Characterization of a short isoform of the kidney protein podocin in human kidney
The podocin short isoform interacts with known podocyte proteins. CD2AP, TRPC6, neprin and NEPH1 co-precipitate with both podocin isoforms (FL, full length (canoncical isoform); SI, short isoform). FLAG- and V5-tagged proteins were expressed in HEK293T cells and precipitated with anti-FLAG antibody as indicated. Western blot analysis was performed with a V5 specific antibody. Expression levels of FLAG.podocin constructs in the lysates are shown below.