Effect of volume expansion with hypertonic- and isotonic saline and isotonic glucose on sodium and water transport in the principal cells in the kidney

Table 2 Effect of 0.9% isotonic saline (0.9% NaCl), 3% hypertonic saline (3% NaCl) and isotonic glucose (Glucose) on urinary output (OU), free water clearence (C _H2O ), urine osmolarity (u-osm), urinary aquaporin2 excretion rate (u-AQP2) and urinary aquaporin2 corrected for creatinine (u-AQP2 _CR ) in a randomized, crossover study of 23 healthy subjects

Periods	Baseline	Infusion	Post infusion			p (GLM-within)
Periods	0-90 min	90-150 min	150-180 min	180-210 min	210-240 min	p (GLM-within)
UO (ml/min)
0.9% NaCl	6.90(1.33)	8.75(1.76)	7.95(2.31)	7.39(2.36)	8.12(2.26)	<0.0001
3% NaCl	7.12(1.35)	2.75(0.73)	1.81(0.64)	1.98(1.07)	2.05(1.09)**
Glucose	6.83(1.58)	11.84(2.28)	16.05(2.82)	15.28(2.47)	13.45(2.47)**
p (GLM between)		<0.0001
p ANOVA	0.77	<0.0001	<0.0001	<0.0001	<0.0001
C _H2O
0.9% NaCl	3.87(1.55)	5.29(1.44)	4.07(2.09)	3.01(2.04)	3.62(1.59)	<0.0001
3% NaCl	3.97(1.21)	-0.35(0.73)	-1.87(0.83)	-2.22(0.88)	-2.26(0.96)
Glucose	3.84(1.32)	6.38(1.77)	11.60(0.52)	12.47(2.35)	11.17(2.12)
p (GLM between)		<0.0001
p ANOVA	0.95	<0.0001	<0.0001	<0.0001	<0.0001
U- osm (mosmol/kg)
0.9% NaCl	140(62)	115(21)	149(58)	176(50)	161(33)	<0.0001
3% NaCl	136(37)	342(96)	606(114)	637(105)	640(111)*
Glucose	136(41)	133(21)	79(21)	52(12)	48(7)*
p (GLM between)		p <0.0001
p ANOVA	0.95	<0.0001	<0.0001	<0.0001	<0.0001
u-AQP2 (ng/min)
0.9% NaCl	1.03 (0.25)	1.02(0.22)	1.22(0.34)	-	1.26(0.32)*	<0.0001
3% NaCl	1.02(0.22)	0.99(0.31)	1.28(0.30)	-	1.37(0.34)*
Glucose	1.01(0.22)	1.26(0.25)	1.19(0.29)	-	0.78(0.18)*
p (GLM between)		0.274
u-AQP2 _CR (ng/mmol)
0.9% NaCl	102.5(19.9)	110.8(18.1)	119.8(25.4)	-	125.0(21.0)**	<0.0001
3% NaCl	105.9(15.7)	108.6(27.2)	125.1(31.4)	-	132.7(26.5)*
Glucose	105.7(19.1)	137.1(22.3)	118.1(26.5)	-	87.9(15.4)*
p (GLM between)		0.565

Values are mean with SD in brackets. General linear model (GLM) with repeated measures was performed for comparison within the group and intervention as between subjects factor. One way ANOVA was performed when differences were found between interventions. Paired t-test was used for comparison within treatment group at baseline and post infusion period 210 – 240 minutes. *p < 0.0001; **p < 0.001.

ISSN: 1471-2369