Figure 3

Representative microphotographs of immunohistochemical examination of renal cortical sections from sham operated and PNx rats. In sham-operated control kidneys the specific staining for both mitochondrial proteins – MCAD and GRP-75 - is distributed evenly throughout the cortical tissue. After PNx, many tubules (especially in fibrotic and inflamed lesions) showed a marked decrease in expression of mitochondrial MCAD and GRP-75 proteins. Bar = 100 μm. The images were taken on a Nikon Eclipse 80i microscope equipped with a Nikon camera HeadDS-Fi1 (Nikon, Tokyo, Japan).