Figure 2

Effect of indoxyl sulfate on phosphatidylserine exposure and hemolysis. A. Original histogram of annexin-V-binding of erythrocytes following exposure for 48 hours to Ringer solution without (-, grey) and with (+, black) presence of 600 μM indoxyl sulfate. B. Arithmetic means ± SEM (n = 24–25) of erythrocyte annexin-V-binding following incubation for 48 hours to Ringer solution without (white bar) or with (black bars) presence of indoxyl sulfate (5–600 μM). For comparison, arithmetic means ± SEM (n = 5) of the percentage of hemolysis is shown as grey bars. *,*** (p < 0.05, 0.001) indicates significant difference from the absence of indoxyl sulfate for the respective measurements (ANOVA).