Figure 4

Effect of Ca ²⁺ withdrawal on indoxyl sulfate-induced annexin-V-binding. Arithmetic means ± SEM (n = 10) of the percentage of annexin-V-binding erythrocytes after a 48 hours treatment with Ringer solution without (white bar) or with (black bars) 150 μM indoxyl sulfate in the presence (left bars, + Ca) and absence (right bars, - Ca) of calcium. *** (<0.001) indicates significant difference from respective control (absence of indoxyl sulfate) (ANOVA) ### (p < 0.001) indicates significant difference from the respective values in the presence of Ca²⁺.