Relative expression of the catalytic alpha (α) domain of AMPK in proximal tubular cells derived from α1
knockout (KO) mice. The relative expression of the α1 and α2 isoforms of AMPK, as well as of the total α domain (α1 + α2), was evaluated in immunoblots of lysates from confluent mouse proximal tubular (MPT) cells derived from the kidneys of either α1-/- or α2-/- KO mice, or their WT controls. Upper panel: Representative immunoblot. Molecular weight markers (in kDa) are shown on the right of each immunoblot. Lower panel: Densitometric quantitation of three immunoblots using β-actin as a loading control. * p < 0.02, α1-/- vs. WT mice, for α2 isoform expression; # p < 0.02, α2-/- vs. WT mice, for α1 isoform expression.