Figure 1

Gating strategy used for the identification of circulating mDCP, pDCP, and tDCP by flow cytometry. Region R1 white blood cells (WBC) were separated from debris and platelets using their forward and side scatter (FSC and SSC). Region R2 was used to exclude granulocytes by SSC, B lymphocytes by CD19 staining, monocytes by CD14 staining, and dead cells by propidium iodide-staining. In regions R3 and R4, circulating mDCP and pDCP were detected according to their specific BDCA-1 and BDCA-2 staining, respectively.