Figure 3

Intracellular Ca ²⁺ release mediates PC2 translocation to the PM. ( A-B ) Quantitative analysis of a number of PM PC2-positive rPT cells following treatment with 1 μM ionomycin ( A ), 1 μM thapsigargin (Tg) ( B ), or thapsigargin with 10 μM BAPTA ( B ). ( C ) Single cell Ca²⁺ recording of two rPT cells loaded with Fura-2/AM treated with 100 μM ouabain followed by sequential addition of 2 mM Mn²⁺ to the recoding medium. ( D ) Statistical analysis of slope decrease in non-oscillating (gray bar) and oscillating cells (black bar) in response to ouabain after Mn²⁺ addition to the recording medium. Values are the mean ± SEM, *P < 0.05, ***P < 0.001 vs. control, and #P < 0.001 vs. treatment.