Figure 6

Immunohistochemical detection of CD31 , MCP-1, CD105 and HIF-1a. Normal group, CD31-positive endothelial cells were regularly distributed in renal interstitium (A); The expression of CD31 was significantly decreased in IgAN group (B); In EPCs group, the expression of CD31 was also decreased than the normal group,however, it was significantly increased compared to the one in IgAN group. (C). In normal group, MCP-1 staining was barely detectable in renal tubular epithelial cells (D); However, in IgAN group, the enhanced expression of MCP-1 was detected. (E); MCP-1 expression gradually decreased in EPCs group (F). Normal group, CD105 staining was weak. (G); IgAN group, the expression of CD105 was significantly elevated. (H); CD105 expression in EPCs group after transplantation. (I). In normal group, minimal HIF-1α was detected in renal tubular epithelial. (J); The expression of HIF-1α increased significantly in IgAN group, (K); It gradually decreased in EPCs group (L). Immunohistochemistry of CD31 (A, B, C); Immunohistochemistry of MCP-1 (D, E, F); Immunohistochemistry of CD105 (G, H, I); Immunohistochemistry of HIF-1α (J, K, L).