Figure 5
Benazepril treatment inhibited the high glucose induced TGF-β 1 , ILK and α-SMA expression at 48 h. (A) The TGF-β1, ILK and α-SMA mRNA expression in NG, MG, HG and ACEI were determined by real-time RT-PCR at 48 h. (B) Western blotting determined ILK and α-SMA protein expression in the NG, MG, HG and ACEI at 48 h. (C) A representative western blot analysis. Densitometric quantification of the corresponding bands is performed using Kodak 1D Image software. Values are means ± SE and are expressed relative to the control. n = 6. *,P < 0.05 vs control; #, P < 0.05 vs DN.