Figure 6

Impact of miR-155 and miR-146a on HRGECs. A, B. The expression of miR-155 and miR-146 under high glucose conditions of different times compared with the control and mannitol group; C, D. Quantification of miR-155 and miR-146a mRNA expression levels with different treating methods on HRGECs by a real-time PCR; E, F. Western blot was used to assess the protein levels of TNF-α and TGF-β with the same treating methods in C and D, glucose group is compared with mannitol group; the high glucose + miR155 inhibitor and high glucose + miR155 mimics are compared to high glucose + scrambled miRNA. High glucose + PDTC is compared with high glucose + miR-155 + PDTC group; G, H. Extracted nuclear proteins were incubated with a biotin-labeled NF-κB probe to detect the DNA-binding activity of NF-κB. Lane 1, normal control group; lane 2, high glucose (25 mmol/L); lane 3, high glucose + miR-155/146a mimics (50 nmol/L); lane 4, high glucose + miR-155/146a inhibitor (100 nmol/L); lane 5, high glucose + PDTC (100 umol/L); lane 6, high glucose + miR-155/146a (50 nmol/L) + PDTC (100 μmol/L). *p < 0.05.