Figure 2

Double immunofluorescent staining to Ki-67, caspase-3 and DAPI of healthy and CNF kidneys: A. Healthy kidney cortex shows Ki-67-positive proliferating cells (arrows) in glomeruli (g), Bowman’s membrane (bm) and interstitium (i), but not in the proximal tubules (pt) B. Nuclear DAPI stain. C. Merging of A + B shows green proliferating and blue non-proliferating cell nuclei. Immunostaining to Ki-67 and DAPI, scale bar 25 μm D. CNF proximal tubules cysts of different diameters: apparently normal tubules (PTNC), cysts with simple squamous epithelium (CS) or cuboidal epithelium (CC) displaying numerous Ki-67- positive cells (arrows) E. Nuclear DAPI stain. F. Merging of D + E shows green Ki-67-positive cells (arrows) and blue non-proliferating cells in epithelium and interstitium. Immunostaining to Ki-67 and DAPI, scale bar 25 μm G. CNF kidneys show apoptotic caspase-3-positive cells (arrows) in dilated proximal (PTC), distal tubules (DT), and interstitium (i). H. Nuclear DAPI stain. I. Merging of G + H shows green apoptotic nuclei (arrows) and blue non-apoptotic nuclei. Immunostaining to caspase-3 and DAPI, scale bar 10 μm.