Figure 1

Area measurement of IgA deposition in a glomerulus. As an example, an immunofluorescence image of a renal biopsy specimen from an IgAN patient is shown. a. The brightness of the immunofluorescence photograph was adjusted by image J software and the edge of glomerulus was traced. b. The color photograph was converted to binary data and the edge of glomerulus was traced. Area-IgA (%) was calculated by the following formula. Area-IgA (%) = (black pixel number) / (whole pixel number within the traced area) × 100. The Area-IgA was 9.8%.