Figure 4
Representative images of histology (first row) and immunohistochemical detection of 3-NT (second row) and DNP (third row) during the evolution of the kidney damage produced by K2Cr2O7. The study was performed in control rats (day 0) and on days 2 and 12 after a single injection of K2Cr2O7 (15 mg/Kg). (A) Normal kidney histology from control rat (H/E). (B) On day 2, there is extensive tubular damage manifested by swollen and necrotic epithelial cells (H/E). (C) total kidney regeneration has been produced on day 12 post K2Cr2O7 administration (H/E). (D) Normal kidney from control animals shows scarce 3-NT immunoreactivity. (E) On day 2, there is strong 3-NT immunostaining in the epithelium from the convoluted tubules. (F) Slight 3-NT immunostaining is observed in the tubular epithelium on day 12. (G) Normal kidney from control rat shows scarce DNP immunostaining in the tubular epithelium. (H) In contrast, on day 2 there is clear DNP immunostaining in the cytoplasm and nucleus of the tubular epithelial cells and mesangium. (I) Slight DNP immunostaining is observed on day 12. 400 X.