Figure 5

Immunohistochemistry for MCP-1 and the macrophage marker ED-1. Panel A-D: examples of photomicrographs of MCP-1 staining (brown) in glomeruli, hematoxylin counterstain (blue nuclei) Panel A (glomerulus of a normotensive normoglycemic rat) represents score 0, panel B (from a normotensive diabetic rat) score 1, panel C (from a hypertensive normoglycemic rat kidney) score 2, and panel D (from a hypertensive diabetic rat) score 3. Panel E, High power magnification of MCP-1 immunohistochemistry (brown) in a hypertensive diabetic rat. MCP-1 staining localized to spindle-shaped cells, probably fibroblasts, surrounding tubules in a double immunohistochemistry with the macrophage marker ED-1 (blue cytoplasm, arrows), methyl-green counterstain. Macrophages were often localized in close proximity to the MCP-1 positive cells surrounding tubules (asterisk). Scale bars (50 μm) are indicated. Note the identical scale for panels A-D but different scale for panel E.