Figure 5

Levels of p21 are inversely proportional to MDCK cell proliferation. a. MDCK cells were seeded 24 h before transfection, and transfected with antisense p21 (AS) or scrambled sequence control (SC) ODN in serum-reduced medium as described in Methods. Cell lysates were immunoblotted with anti-p21 and β-actin antibodies. GJ: Gene-Juice (transfection reagent); opti: Optimem medium. b. MDCK cells transfected with antisense p21 or control ODNs were incubated with MTT and purple soluble formazan was assessed as described in Methods. Experiments shown are representative of at least two experiments.