Figure 5

Immunoperoxidase staining demonstrating similar levels of nitrotyrosine in podocytes of PodoTrsp ^-/- and control ( Trsp ^L/L ) mice. Consecutive 3 μm kidney sections from vehicle control, vehicle KO (PodoTrsp^-/-), STZ control, and STZ KO (PodoTrsp^-/-) mice were stained for nitrotyrosine or Wt1 (to identify podocytes). Arrows indicate selected podocytes in the Wt1 and merged images, and indicate the identical positions in the nitrotyrosine images. Negative control staining for Wt1 utilized normal IgG in place of the primary antibody, and for nitrotyrosine utilized coincubation of the primary antibody with nitrotyrosine.