Figure 2
Reaction velocity ratios plotted against uremic solute (sample) concentrations in the crocin bleaching assay. (A) Trolox; (B) uric acid; (C) p-cresol; (D) phenol; (E) L-tyrosine. [C], crocin concentration and [A], sample concentration. The inset shows the decrease in absorbance of crocin, at 443 nm, over 10 min: (v0) velocity in the absence and (v) velocity in the presence of various concentrations of samples. The experimental conditions are described in Methods. The ROO• scavenging assay was performed in duplicate testes.