Table 2 Primary antibodies used in the immunohistochemistry
Target Molecule | Immunogen | Host | Type | Source | Dilution |
|---|---|---|---|---|---|
AGT | Human AGT | Mouse | Monoclonal | R&D Systems | 1:600 |
AngII | Human angiotensin II | Rabbit | Polyclonal affinity purified | Novus Biologicals | 1:100 |
Ang-(1-7) | Rat angiotensin (1-7) | Rabbit | Polyclonal affinity purified | Alomone Labs | 1:100 |
ACE | Human ACE | Mouse | Monoclonal | Abcam | 1:50 |
ACE2 | NS0-derived rhACE-2 ectodomain | Goat | Polyclonal affinity purified | R&D Systems | 1:10 |
Chymase | Human chymase (skin) | Mouse | Polyclonal affinity purified | Chemicon International | 1:500 |