Fig. 1

The number of cells of renin lineage (CoRL) increases beyond the JG location following UUO. CoRL were identified by RFP reporter staining (red), endothelial cells were labeled with CD31 staining (green). Nuclei are labeled with DAPI (blue). (a–g) CoRL in Cortex. (a) Representative images show that in sham-operated mice, labeled CoRL (arrowheads) are predominantly localized to the juxta-glomerulus, with fewer detected along afferent arterioles (arrow). (b) At d7 post UUO, occasional labeled CoRL were detected in glomerular capillary loops. (c) Labeled CoRL were rarely detected in peritubular locations at day 14. Quantification: (d) Total cortical CoRL number was not statistically significant following UUO, (e) the same was found when CoRL were quantified exclusively in JG compartment. However, (f) there was a significant increase of intraglomerular CoRL at d3, and (g) peritubular CoRL at d7 and d14. (h–k) CoRL in Medulla. (h) Quantification - total CoRL number in the medulla was significantly increased on day 7 following UUO. (i) In the medulla of the sham kidney, CoRL were restricted to vasa recta. (j) Following UUO there was an increase of CoRL in vasa recta on day 7 (arrowheads). (k) 14 days after UUO, a subset of CoRL localized away from any vessels, being present in the interstitium